Epifluorescence microscopes

We have three different epifluorescence microscopes in combination with other functions.
Epifluorescence microscopy is a method of fluorescence microscopy that is widely used in life sciences. The excitation light is passed from above (or, for inverted microscopes, from below), through the objective and then onto the specimen instead of passing it first through the specimen. The fluorescence in the specimen gives rise to emitted light which is focused to the detector by the same objective that is used for the excitation. Since most of the excitation light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and this method therefore gives an improved signal to noise ratio. An additional filter between the objective and the detector filters allows to eliminate remaining excitation light from fluorescent light.

ECLIPSE ME 600 (Nikon)
This is an upright epi-fluorescence microscope with attached Ocean Optics Fibre- Spectrometer. Dark and bright field observation is possible. The instrument is situated in the surface science laboratory. This instrument is used, e.g., for morphologic and spectroscopic studies of light emitting organic thin films.
ECLIPSE TE 300 (Nikon)
This instrument is an inverted epi-fluorescence microscope with phase contrast option; it hosts the JPK AFM (Nano Wizard) and is situated in our microscopy laboratory.
ECLIPSE TE 2000-U (Nikon)
An inverted epi-fluorescence microscope including femtosecond laser scanning microscope option. This instrument is situated in our nanooptics laboratory.

Company link